Microbiology Procedure
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Air Centrifuge

Air Centrifuge - The first primitive type of air centrifuge was developed by Wells (1993). The principle of air centrifuge is that the particles from air are centrifuged onto the culture medium. In his air centrifuge sampled air was passed along a tube which was rotated rapidly on its long axis. The inner surface of the tube was lined with culture medium and any bacteria containing particle deposited on it grew into a colony on incubation.

A modern version of this centrifuge is the Reuter centrifugal air sampler, which is portable and battery powered. It resembles a large cylindrical torch with an open ended drum at one end. The drum encloses impeller blades which can be rotated by battery power when switched on. A plastic strip coated with culture medium can be inserted along the inner side of the drum.

Air is drawn into the drum and subjected to centrifugal acceleration. This causes the suspended particles to impact on the culture medium. After sampling the strip is removed from the instrument and incubated at 37°C for 48 hours. Later the colonies can be counted.

Advantage of this sampler is that it is very convenient for transportation and use. The disadvantage is that it is less efficient than the slit sampler in detecting particle below 5mm in diameter. More over the size of the air being sampled cannot be accurately controlled.

 

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