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Air Sanitation by Ultraviolet Radiation

Air Sanitation by Ultraviolet Radiation

Radiation of short wave lengths are more powerful in controlling airborne microorganisms. Among these ultraviolet (UV) is the widely used one. Ultraviolet radiation has a wavelength range of 210-328 nm. The maximum microbicidal effect of UV light is considered to be around 260nm. This wavelength is also the peak absorption wavelength for DNA.

Modem low pressure mercury vapour lamps emit more than 95% of their radiation at 253.7nm and this is at the maximum microbicidal activity. Thus about 50% of the total energy input to the lamp is transmitted as effective UV radiation through the special glass in the lamp. About 2% is transmitted into visible light. The remaining 48% is transformed into heat.

Target sites and microbial inactivation mechanisms:
The main target site for UV radiation is the DNA. Various photo products accumulate in UV exposed microbes. Exposure of non sporulating bacteria to UV radiation results in the formation of purine and pyrimidine dimers between adjacent molecules in the same strand of DNA. UV also induces nucleic acid - protein cross links.

In Deinococcus radiodurans another type of photo product, 5, 6 - dihydroxy dihydrothymine, has been found on exposure to UV radiation. In bacterial spores yet another type of photo product, 5 thyminyl 5-6 dihydro thymine (TDHT), accumulates in DNA. Unless removed, these photo products form non coding lesions in DNA which ultimately lead to cell death.

 

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