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Index >> Air Microbiology >> Collection and Enumeration of Aeroallergens

Collection and Enumeration of Aeroallergens

Collection And Enumeration of Aeroallergens - Various methods have been developed to collect and quantify the pollen and fungal aeroallergens. Durham's gravitational sampler, developed in 1940s is the simplest and most commonly used sampler even today in some parts of the world including India. It is a simple device consisting of two metallic discs separated by three struts.

The microscope slide coated with adhesive is mounted an inch above the centre of the lower disc, upper disc acting as rain shield. The slides are exposed for 24 hr. The sampler can be used for qualitative analysis of various kinds of spores, particularly larger ones, giving a rough idea of seasonal trend.

However, some sophisticated samplers based on impaction and suction devices (battery - as well as power-operated) have now been developed. These provide more accurate data on species-composition as well as density of aeroallergens. Based on suction device with definite amount of air sucked in and impacting the bio particles on the adhesive material on the sampler is the most acceptable device world over.

The Burkard volumetric traps and Rotorod aeroallergen models are such samplers. Burkard personal slide sampler (suction), battery operated, suitable for indoor environment is 10 cm high having a rectangular orifice at the top. The micro slide is coated with glycerine jelly. The sampler sucks air at flow rate of 10/minute through the orifice.

The particles get impacted on the slide forming a band. Slide is mounted and scanned. Burkard personal Petri dish sampler is similar to the slide sampler except that it has a stage to hold a Petri dish and a sieve to cover it. The orifice is circular. Petri dish containing suitable growth medium become exposed to a definite volume of air through the sieve on to the Petri dishes. These two samplers arc suitable for indoor environment. Burkard continuous 7 day trap (continuous impaction) uses a glycerine jelly coated tape mounted on a rotating drum.

The drum is conn­ected to a timer and rotates at a constant speed. Tape exposed for a week is cut into seven strips, one for each day, mounted on a slide in glycerine jelly and scanned. Each strip can also be divided into 24 vertical bands, each band representing the particular hour of the day.

 

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