Microbiology Procedure
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Index >> Air Microbiology >> Hazardous Procedures

Hazardous Procedures

Hazardous Procedures - The procedures and situations which may pose a risk of infection include the following:Withdrawal of needle from a vein or culture may liberate aerosol on vibration.

When the content of a syringe is forcefully ejected splashing or spraying may result. If the last drop from a pipette is blown out, the infective material may be disseminated into the surrounding in drips or aerosols.

Infective aerosol may be produced when vibration occurs in a long inoculation wire; when a wet inoculation wire is flamed; when a hot inoculation wire is cooled and a smear is made on a slide for staining.

Shaking of cultures may produce aerosol within a container and if the container is opened within a period of time it may liberate the aerosol. Spillage is also possible when cultures are mixed or shaken.

During centrifugation of culture fluids vibration can generate aerosol within the container and the aerosol may be released on opening.

During freeze drying of cultures contamination of the environment may occur. Similarly opening of the sealed ampoules may produce aerosol.

Even with safety cabinets dissemination of infection inside the room is possible if they are not used in a proper way (Exhaust ducts from the cabinets should be situated so as to avoid the re-entry of used air into the room).Animal cages may become contaminated with discharges and excreta.. When disturbed these may liberate infectious dust.

Removal of cotton plug which is wet with the culture fluid may produce aerosol. If it is dry it may lead to the production of infective dust.Accidental dropping of culture plates, tubes or bottles may liberate aerosols.

 

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