Microbiology Procedure
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Lemon Sampler

Lemon Sampler - It consists of a glass Folin aeration tube with a perforated bulb with six holes at one end. The bulb end of aeration tube is contained within a test tube by a two hole rubber stopper. The bulb is actually centered near the bottom of the tube and is immersed in 20ml of broth. Two or three drops of olive oil is added to the broth to prevent foaming.

A kjeldahl trap with square glass baffle is shortened at both ends for convenience. The intake end is slightly bent and inserted into the other hole of the stopper. A flow meter measures the rate of airflow entering the upper open end of the Folin tube. An air pump is attached to the exhaust end of the kjeldahl trap.

The entire bubbler should be sterilized by autoclaving. Alternatively it can be sterilized by rinsing with 70% alcohol and dried afterwards. Air is drawn at the rate of 25-30 liters per minute and dispersed through the broth.

 

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