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Index >> Animal Viruses >> Orthomyxoviruses

Orthomyxoviruses

Orthomyxoviruses - The orthomyxoviruses are enveloped viruses with helical nucleocapsids 6-9 nm in diameter and of variable length. The particle is 80-120 nm in diameter and its shape is pleiomorphic. The influenza virus is a representative of orthomyxoviruses. Three strains of the influenza virus, A, Band C cause influenza in humans. The AI strain is the most important because it undergoes constant antigenic variation.

The nucleocapsid of orthomyxovirus is ribonuclease sensitive and thus differs from the nucleocapsids of other non segmented viruses. It shows a pseudohelical morphology. According to one model it consists of two helical protein strands (along with RNA). The nucleocapsid is enclosed within an envelope. The internal ribonucleoprotein (RNP) is normally not visible in the intact virion

The surface of the envelope is covered with spikes arranged with regular spacings. According to one model, the approximately spherical envelope has hexagonally spaced spikes. Twelve of the spikes would be surrounded by neighbours and the remainder by 6 neighbours. The envelope consists of a lipid bilayer with which are associated proteins.

The spikes consist of glycoprotein. There are two types of glycoproteins, one showing haemagglutinin activity and the other neuraminidase activity. Removal of the spikes by treatment with proteolytic enzymes renders the viral particles noninfectious. Internal to the lipid bilayer is a major protein called the M protein (for 'matrix' or membrane protein).

It probably stabilizes the, lipid bilayer by providing a more rigid framework to the particle. The genome consists of negative strand (anti mRNA) ssRNA 10,000-13,000 bases long. Each gene, however, occupies a separate segment, as in diplornaviruses, The segmented genome permits high levels of recombination between genes located in different segments.

RNA segments of human influenza viruses and animal influenza viruses are subjected to constant shifting, leading to constant variation 10 the properties of the Virus. The Virion RNA segments transcribe, complementary mRNA with the help of an RNA dependent RNA polymerase (replicase), which is an endogenous viral enzyme

Although the evidence is conflicting, it is likely that transcription in the influenza virus occurs in the nucleus. Two other groups of enveloped negative strand viruses, the Bunyaviruses and the Arenaviruses, employ the replicative strategy of orthomyxoviruses.

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