Environmental Factors Affecting Denitrification,Organic Matter,Nitrogen,Pseudomonas Aeruginosa,Micrococcus Denitrificans,Immobilisation

Environmental Factors Affecting Denitrification

	Environmental Factors Affecting Denitrification Rate of denitrification is far more slow in soils low in carbon than on land that is rich in organic matter. When soil is waterlogged, loss of nitrogen is more when compared to well-drained soils which is due to the immobilisation of inorganic nitrogen. Aeration affects the transformation in two ways: Denitrification proceeds only when the oxygen supply is insufficient to satisfy the microbiological demand.
At the same time, oxygen is necessary for the formation of nitrites and nitrates which are essential for denitrification. For example, in submerged soils used of rice cultivation, the ammonium is oxidised to nitrate in the oxygen containing surface layer and nitrate is converted to gaseous products of denitrification as it diffuses into the underlying anaerobic zone. Many of the bacteria that bring about denitrification are sensitive to high hydrogen ion concentration, hence acid soils contain a sparse denitrifying population.
The optimum temperature for the reaction is 25°C-60°C. The enzyme involved in denitrification is nitrate reductase or nitratase. This enzyme is found in E.coli, Pseudomonas aeruginosa, Micrococcus denitrificans.