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Index >> Applications of Microbial Interactions >> Sources of Organic Carbon

Sources of Organic Carbon

Sources of Organic Carbon
Carbohydrates These are the most common types of organic carbon entering the soil. The most abundant organic compound on earth is cellulose, which provides the primary structural component for plants. (Chitin, present in insects, crustaceans, and bones, is the second most abundant organic compound.) Like starch, cellulose is a polymer of glucose monomer units, linked together at the beta-l,4 Iocations as opposed to the alpha-l,4 locations for amylose (insoluble starch).

Enzymes are generally extremely specific in their catalytic actions. They can recognise even the subtlest difference in the substrate structure and often exhibit no measurable catalytic behaviour toward other similarly structured substrates.

The difference in the glucose linkage between starch and cellulose makes it impossible for the starch digesting enzymes, e.g. alpha-amylase, to break down cellulose. The direct consequence of this specificity is that various organisms, including humans, cannot use cellulose to satisfy their nutritional requirement for carbohydrates.

However, some animals and insects, such as cattle, sheep, horses, termites, and caterpillars, can subsist on wood and grass, although they themselves do not produce cellulolytic enzymes. This is due to the synergistic effect of the bacteria present in their digestive tracts. These gut bacterial flora secrete the necessary cellulolytic enzymes to digest cellulose, and the hosts, in turn, provide them with a shelter as well as nutrients.

Sugars Monosaccharides are simple sugars which are building blocks and universal energy substrates for soil microbes. Disachharides are present mostly in fungal cell wall materials.

Lignin It is second to cellulose in terms of biomass. It is the structural component of plant which imparts rigidity and resistance to compression, bending and pathogens. It protects cellulose and hemicellulose from enzymatic attack. It is a cementing agent, amorphous and highly branched with no defined structure.

It is a complex polymer of coumaryl, coniferyl subunits. It is an aromatic ring (phenyl) with a three-carbon side chain (phenyl propanoid). Lignin is the richest source of aromatic (carbon) compounds in nature.

Fats, waxes and hydrocarbons They are carbon compounds soluble in ether. Fatty acids are components of cell membranes. They are either saturated or unsaturated (contain one or more double bonds between carbon atoms). Some of the problems associated with microbial utilisation of plant carbon include physical barriers by cell wall compounds, cutins, suberins, chitins etc. lignin which slows the rate of decomposition by microbes, and the microbicidal properties of some plants.

Water is required for hydrolysis and to increase surface area for enzymatic degradations. Plants contain a mixture of chemical components like cellulose, hemicellulose, pectin, lignin, waxes and their decomposition requires combined action of many microbes, none of which have all the enzymes required to completely decompose the whole plant material. Any environmental factor affecting the soil biota affects the decomposition also.

For example, decomposition of organic matter is rapid in tropical regions when compared to temperate since the temperature there is warmer and microbes have a high metabolic rate.

Substrate quality has an effect on decomposition which depends on C : N ratio, if C : N ratio is greater than 20-30 : 1, then immobilisation of nitrogen results and consequently decomposition of carbon slows down. For example, lignin content slows down the rate of decomposition because it has no nitrogen.

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