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Flow Cytometry

Flow Cytometry- Flow cytometers or flourescence activated cell sorters are instruments which analyze optical parameters of many individual cells in a short space of time. The sample flows through an orifice and across the path of a light source, usually a laser. Optical parameters like light scatter and fluorescence are measured on each cell by photodetectors. The signals received are processed by a computer. On the basis of predefined optical properties cells can be sorted and collected automatically. Some of the advantages of flow Cytometry are:

I. Rapid analysis of relatively large sample sizes; i.e., 104-106   cells per minute.

2. Modifications of the technique allow detection of smaller and less abundant cells in natural water samples.

The well known utility of flow cytometer is the analysis natural phytoplankton by autofluorescence. By this technique cyanobacterial phototrophs are enumerated and separated from other phytoplankton based on phycoerythrin and chlorophyll fluorescence. The former fluoreces orange while the latter red.

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