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Fungal Cell Wall Composition

Fungal Cell Wall Composition  -

Electron microscopy shows that the cell wall of the yeast Saccharomyces spp., is composed of two or more layers.

The wall contains about 29 per cent glucan, 31 per cent mannan, 13 per cent protein, 8.5 percent lipid and three percent ash.

Yeast cell walls contain three protein complexes: glucan protein, mannan protein I and-glucomannan protein II.

Cell walls also contain a peptide polysaccharide (peptidophosphomannan) in which low molecular weight mannose containing oligosaccharides are attached by O-glycosidic linkage to seryl and threonyl residues.

The mannan backbone is composed of (a 1,6) linked mannopyranosyl residues and the mannopyranosyl, mannobiosyl, and mannotriosyl residues are attached by (a1,2) linkage to the backbone.

The cell wall occupies about 40 per cent of the cell volume and has a uniform thickness around the protoplast.

Cell walls of yeast can be removed without destroying the protoplast by the use of specific enzymes that hydrolyse the cell wall.

Cell walls of mycelial fungi contain mostly chitin which is a polymer of n-acetylglucosamine and may constitute 25-30 per cent of the dry weight of the cell.

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