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Main Index >> Bacterial Recombination DNA Technology

Types of Bacterial Recombinations
Mechanism of Bacterial Recombination
Breakage and Reunion
Breakage and Copying
Complete Copy Choice
Rapid Lysis Method by Hershey
Host Range Method by Luria
Copy Choice Method by Belling
Models of the Molecular Basis of Generalised Recombination
Meselson and Radding Model
Strand Displacement
Strand Uptake
Loop Cleavage
Assimilation
Isomerization
Branch Migration
Propagation of Strand Transfer
Assimilation and Asymmetric Strand Transfer
Nonreciprocal Recombination
Transposable Elements
IS ( Insertion Sequence ) Elements
Mutations
Tn ( Transposons ) Elements
Mechanism of Transposition by Berg
 

Bacterial Recombinant DNA Technology


It is genetically possible to "tailor" the microorganisms for the production of any microbial metabolite vitamin, amino acid or enzyme. Gene cloning extends the genome of the microorganism by allowing the introduction of novel genes from comparatively unrelated species. The cloning of genes from higher eukaryotes, particularly from man and his domestic animals, has been seen to offer even greater industrial potential. Which microbes should then be used as universal recipients for such genes and hence as production organisms. The two most ideal are the pro­karyote, Escherichia coli and the eukaryote, Saccharomyces cerevisiae.

Some proteins could be obtained on large scale through fermentation by methods, relatively more cheaper than the conventional ones. For example, human growth hormone was previously extracted from the pituitary gland of cadavers and was mostly in short supply. Now, increase in supply should help more patients. Equally important is the development of new vaccincs through gene cloning. Genes for single antigens can be cloned and expressed by bacteria and a purified antigen which has not been derived directly from the pathogenic organism or virus may be used as a vaccine. In this way, vaccines for viral hepatitis and foot and mouth disease have been developed.

 

Transformation
Competence
Binding
Penetration
Synapsis
Integration
Conjugation
Unidirectional Genetic Transfer
Properties of Sexual Mating Types
F Factor
Infectious Transfer of F Factor
Interaction Between F Factor and Chromosome
Chromosome Transfer
Integration of Donor DNA
Transduction
Generalised Transduction
Role of Defective Phage
Complete and Abortive Transduction
Specialized - Restricted Genetic Transduction
Low Frequency Transduction
High Frequency Transduction