Microbiology Procedure
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Index >> Bacterial Recombination >> Conjugation

Conjugation

Conjugation -  In 1946 Leder berg, a student of Tatum, established in bacteria a process resembling sex. In conjugation there is physical contact between two genetically different bacterial cells of the same or closely related species. This feature distinguishes conjugation from transfor­mation and transduction. A conjugation tube is established between the two conjugants, followed by the transfer of genetic material from one cell (the donor or 'male') to the other (the recipient or 'female')

The conjugation tube is formed by special appendages called sex pili, specified by a plasmid, the F factor, in the donor .The recipient cells appear to have a specific receptor site for Conjugation. It should be noted that there is no exchange of genetic material during conjugation, only unilateral transfer. Bacterial cells normally exist in the haploid state.

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