Integration

Integration

	Integration The un synapsed recipient DNA strand is cut in two places by an endonuclease. An exonuclease then degrades the ssDNA strand. Integration of the donor DNA strand involves a small amount of repair synthesis to close the gaps present, followed by final sealing with a ligase.
The transformation heteroduplex formed undergoes replication to form transformation homoduplexes. One of these is a normal duplex, while the other is a transformed duplex. The normal duplex is all recipient in origin and the transformed duplex all donor. The clone produced from the transformed duplex is the transformed one. The normal duplex will give rise to a non transformed clone.