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Low Frequency Transduction
The lambda prophage can undergo exchange with the nearby gal segment of bacterial DNA. The vegetative lambda now contains a segment of the bacterial chromosome including gal. A part of the phage genome (the h region) is transferred to the bacterial chromosome in this exchange. The particle is defective and carries gal, and is hence known as λdg (for lambda-defective-gal). Such phage-bacterial chromosome exchange takes place only in an occasional cell. Lambda dg particles constitute only a minute fraction of the lysate particles. The lysate is, therefore, called allow-frequency-transduction (LFT) lysate.