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Mechanism Of Transposition Byberg
Berg (1977) has proposed a mechanism for the transposition of the Tn5 element. This element determines resistance to the antibiotic kanamycin, and can be transposed from the lambda phage to the E.coli chromosome, and from one chromosomal site to another. The frequency of transposition on the E. coli chromosome is between 10-2 to 10-3. There are many potential sites for the insertion of Tn5. The lacZ gene alone contains 19 sites. The revertibility of Tn5 mutations on lacZ gene is 19 out of 20, indicating that there is no loss of recipient DNA sequences during insertion.