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Properties Of Sexual Mating Types

Properties Of Sexual Mating Types
The F factor confers certain surface properties to F+ and HFr cells which enables them to act as donors. These properties do not appear to be influenced by whether the F factor is in the free or integrated state. Loeb (1960) observed that certain bacteriophages can act as donors. This property of male-selective specificity is possessed by the RNA phages f2, MS2, R17, M12 and Qβ, and the ssDNA filamentous phages f1,fd and M13

The donor E. coli cells have F pili or sex fimbriae - on their surface, in addition to the type I pilus. Phages like R17 and M12 are adsorbed on the F pili, but not on recipient bacteria. The adsorption of the RNA phages is at random sites along the length of the sex pilus. Male-specific DNA phages bind to the tip of the pilus. The number of F pili on donor cells is much less than the number of type I pili. Often only one F pilus is present per cell, and there are never more than three pili. Under certain conditions of growth there may be up to five pili per cell. There is a correspondence between the number of F pili and the number of copies of the F factor. This had led to the conclusion that each pilus is synthesized under the direction of one F factor

When an F+ strain of bacteria is incubated overnight in a nutrient medium containing acridine orange, it is converted to the F - state. Acridine orange has no such effect on the HFr strains. This has led to the conclusion that curing by acridine orange can be brought about when the sex factor is in autonomous condition, but not when it is in the integrated condition. Acridine acts by inhibiting replication of the autonomous F factor, and is therefore effective only with growing bacteria. The acridine orange test is used to determine whether the F factor in a strain is present in the autonomous or integrated condition.

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