Microbiology Procedure
  Home  Link to us  Resources  Site map  Search  Language

Index >> Bacterial Recombination >> Strand Displacement

Strand Displacement

Strand Displacement -When new synthesis starts at a nick it displaces a strand. The displaced strand then itself becomes a template for the synthesis of a new strand. The first example was reported in E coli, where DNA polymerase I starts at a nick and catalyses the displacement of a strand just ahead of itself. In the phage TS also DNA polymerase promotes strand displacement. In the phage T7 gene 4 protein, a DNA-dependent ribonuc1eoside-5'-triphosphatase promotes strand displacement by unwinding the DNA.

In T4 the gene 32 protein (H DP) stimulates DNA synthesis in vitro by specific interaction with polymerases and enables the DNA polymerase to start new DNA synthesis at a nick presumably by promoting strand dis­placement. In E coli strand displacement resulting from new DNA synthesis is brought about by the joint action of polymerase III rep protein and HDP

Home | Site map | Submit Article | Resources | Search