Index >> Bacterial Structure >> RNA  Protein Interactions

RNA Protein Interactions - Interaction between protein and RNA has been considered to be the key to the understanding of ribosome structure and function"

There is strong support for the view that most, if not all, ribosomal proteins have direct contact with RNA.

5s R NA:It has been shown that 5s rRNA forms a specific complex with proteins L5, L18 and L25 of the 50S ribosomal subunit .

165 RNA: Treatment of the 30S subunit with nuclease yields ribonucleotide fragments. 16S RNA can be divided into three specific regions (Zimmerman, 1974).

Some proteins, e.g. S8 and 815, are associated with short RNA sequences about 50 nucleotides long, while the 84 binding site comprises almost one-third of the 16S RNA molecule.

The initiation factor IF-'3 has some affinity for rRNA. It has been suggested that a segment of 16S RNA provides a binding site for IF-3 close to its 3' end.

16S RNA thus help to mediate an interaction between IF-3 and mRNA.

235 RNA readily splits into 13S and 18S fragments. Individual proteins or groups of proteins can be rebound to these two fragments, enabling localization of the binding sites.