There is a low degree of cross linkage between the glycan strands of peptidoglycan.
In many gram negative bacteria the peptidoglycan layer is a monolayer, or, at the most, a bilayer.
In E. coli, peptidoglycan consists of linear polysaccharide chains consisting of alternating residues of the amino sugars N-acetyl glucosamine (NAG) and N-acetyl muramic acid (NAM) linked by β-1-4-glycosidic bonds.
Free tetrapeptide side chains are attached to the NAM residues.
Some adjacent NAM residues are directly cross-linked by tetrapeptide side chains.
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