Index >> Bacterial Structure >> Proper Initiation of Endospore Germination

Proper Initiation of Endospore Germination - Germination can only take place after activation. This is a rapid process which is marked by loss of heat resistance and resistance to other harmful agents and the initiation of metabolic activity.

Soluble materials to the extent of about 30% Of the dry weight are liberated. The peptidogiycan of the cortex undergoes hydrolysis to peptidoglycan fragments (spore peptides).

The loose cross-linking of cortical peptidoglycan promotes its rapid diges­tion. a-dipicolinate, which confers the heat resistance property, is released from the cells, which now become heat-sensitive.

There does not appear to be significant macromolecular synthesis during germination.

Metabolic activity that is detected is due to the activation of pre-existing inactive enzymes present in the spore protoplast.

Germination may be divided into two stages, the micro lag and the micro germination time, Microlag extends from the addition of the triggering agent to the beginning of loss of refractility .

The micro germi­nation time extends from the beginning of the loss of refractility to the complete loss of refractility.