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Index >> Bacterial Taxonomy >> Gram Negative Aerobic Rod and Cocci

Gram Negative Aerobic Rod and Cocci

This part comprises of bacteria belonging to a large number of taxonomic groups, differing in morphology, physiology and metabolic, processes and includes five important families such as the Pseudomonadaceae, Azotobacteraceae, Rhizobiaceae, Methylomonadaceae and Halobacteriaceae.

The family Pseudomonadaceae includes bacteria which are straight or curved rods and are motile by polar flagella. These bacteria are a versatile group of chemoorganotrophs and have an oxidative metabolism. G +C content ranges between 58-70 moles percent. This family includes organisms that can grow in mineral media with a single organic compound as sole source of carbon and energy. Most pseudomonads are common inhabitants of soil, freshwater and marine environment. A few are pathogenic to plants and some, occasionally are animal pathogens (P. aeruginosa). Some Pseudomonas produce bacteriocins called pyocins. Type genus is Pseudomonas which is represented by a large number of species (about 265). The genus Xanthomonas includes species which are plant pathogens.

The family Azotobacteraceae is characterized by large cells which are predominantly rod shaped to oval and motile by peritrichous or polar flagella . The bacteria are heterotro­phic and capable of fixing molecular nitrogen in a nitrogen free or nitrogen poor medium with organic compounds as energy source. They are strict aerobes and are inhabitants of soil, water and leaf surfaces. The family includes four genera such as Azotobacter, Azomonas, Beije­rinckia, and Derxia

The family Rhizobiaceae consists of bacteria which are rod shaped, motile with one polar or subpolar flagellum or 2.6 peritrichous flagella. The family has two genera such as Rhizobium and Agro­bacterium. Rhizobium species form nodules on roots of legume plants and fix nitrogen within the root nodules, while species of Agrobacte­rium do not produce nodules but cause other types of hypertrophy on many plants and do not fix atmospheric nitrogen. Genus Rhizobium is divided into two sub groups based on flagellation and rate of growth ­on yeast extract media . The species are defined by the host that they infect and nodulate . DNA base composi­tion is 59-65 mole per cent.

The family Methylomondaceae is represented by bacteria utilising one carbon compounds such as methane or methanol as carbon source and are unique in their limited nutritional versatility. It includes two genera Methylomonas and Methylococcus. The former have a G + C content of 52.1 moles per cent while the latter have 62.5 moles per cent. The family Halobacteriaceae is represented by bacteria which are rods and cocci that need a high concentration of NaCL (2M) for growth. A major characteristic of this group is that cell walls do not contain diaminopimelic acid and muramic acid but contain largely lipoprotein. Metabolism is respiratory and little is known about the utilization of carbohydrates. Colonies are of various shades of red, pink, orange, vermilion etc. The family contains two genera namely Dalobacterium and Halococeus. The former is pleomorphic, specially in deficient media, aerobic, motile by a tuft of polar flagella or non­motile. Do not form dormant structures but survive in salt solutions for many years.

The genus Haloeoccus consists of bacteria which are cocci, 0.6-1.5 µm in diameter and occur in pairs, tetrads and irregular clusters of tetrads. They are non-motile, aerobic and grow only in the presence of 2.5 M or higher concentration of NaCl.Among the genera of uncertain affiliation, Acetobacter and Brucella are important. The genus Acetobacter has cells which are ellipsoidal to rod shaped, straight or slightly curved, may be motile by peritrichous flagella or non motile. Metabolism is respiratory and oxidize ethanol to acetic acid. Generally, no pigments are produced. Found on fruits and vegetables, vinegar and cause spoilage of alcoholic beverages.

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