Microbiology Procedure

Index >> Bacteriophages - Part Two >> Transduction in Lambda Phage

Transduction in Lambda Phage

	Transduction in Lambda Phage - As a result of a mistake made during excision, the bacterial bio or, gal gene is included in the progeny phage genome. The frequency of such occurrences is about one in a million. Since the phage capsid can accommodate only a limited amount of DNA, a part of the phage DNA is left out. This produces a chromosome which is deficient in some phage genes.
The phage in hence inactive. Such a phage may inject its DNA into a bacterial cell, and the DNA may be inserted, Into the bacterial genome. The host now has an additional gene from the first bacterial cell is known as specialized given in the chapter on. This process transduction.
The details of transduction are 'Recombination’. Excision is almost always precise. The phage DNA breaks away the from, bacterial DNA exactly at the point of entry. In rare (I in 100,000) abnormal excisions the break occurs in such a way that the circular phage DNA molecule has a part of bacterial DNA, and vice versa. 'Transducing' virus containing phage DNA along with a part of the gal gene has been found.
When lambda DNA is mixed with lambda gal DNA (hybridization), the two DNAs undergo complementary pairing, except in the gal region. In such heteroduplexes the two DNAs remains as separate single strands in the gal region, forming a loop coiled the substitution bubble.

Home | Site map | Submit Article | Resources | Search

Language >> English | Français | Español | Deutsch | Italiano | Portugues | Japanese | Korean | Sim-Chinese