DNA Binding Protein

DNA Binding Protein

DNA Binding Protein - The T4 DNA binding protein is specified by gene 32. It is required for both replication and recombination. Gene 3r mutants are defective in both processes. There are about 10,000 copies of the DNA binding protein per infected cell. This corresponds to more that ISO molecules per replication fork.

The affinity of the DNA binding protein is much greater for denatured DNA than for dsDNA. The protein is therefore able to maintain ssDNA in that state. Its role appears to be to provide a single stranded template for DNA polymerase.

It has been found to stimulate DNA polymerase activity in vitro. The DNA binding protein maintains DNA in the single stranded state ahead of the replication fork. The DNA polymerase thus comes into contact with only single strands as it proceeds with replication

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