Microbiology Procedure
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Index >> Bacteriophages - Part One >> Adsorption of Virion to Host Cell

Adsorption of Virion to Host Cell


Adsorption of Virion to Host Cell
- a.Landing:Infection begins with co1lision between the phage and the E. coli cell brought about by random thermal movements of bacterial cells and phage particles. Contact is made with the phage tail.

The tail fibres recognize specific receptor sites on the host cell wall during attachment. Phage T3, T4 and T7 receptors are located in the lipopolysaccharide (LPS) component; while T2 and T6 bind to the lipoprotein components.

The T5 receptors contain both lipoprotein and LPS. The tail fibres are normally folded around the tail, with their midpoints held by the whiskers on the collar.


When the tail comes into contact with the bacterial cell, the fibres unfold. In the Benzer strain of phage T4 (T4B) the release of the tail fibres from the whiskers depends upon L-tryptophal1 (1 mg/ml) and a cofactor.

The Doerman strain (T4D) does not require tryptophan. The Hershey strain (T2H) is inhibited by indole. Indole causes the tail fibres to align themselves along the tail as they become inactivated. At high concentrations (O.2-0.6M) certain sugars inhibit T4 adsorption to bacterial cells. Glucose inhibits adsorption 100%, but galactose has no effect:


Pinning
. Adsorption occurs in two stages, an early reversible stage and a later irreversible stage. In the early stage of adsorption the phage is attached through the tips of the tail fibres.

The phage moves about on the cell surface, with the fibre tips probably remaining attached, until it comes into contact with a site at which it is pinned through the tailpins or spikes.

Pinning represents the irreversible stage of adsorption. It appears to occur at points where the cell wall adheres to the plasma membrane of the bacterial cell.

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