Chain Elongation,Holoenzyme,DNA Polymerase III,Okazaki Fragments,Viral Strand,Holoenzyme,DNA Polymerase,Multimeric Form, Covalently Continuous

Chain Elongation

	Chain Elongation - A complementary (-) strand is extended on the RNA primer, using the viral (+) strand as the template. Chain elongation takes plaice with DNA polymerase III holoenzyme. The holoenzyme is composed of DNA polymerase III, formed by the dnaE locus, and DNA co polymerase III (MW 77,000).
It appears to be functionally equivalent to DNA polymerase III., plus dnaZ protein, plus factors I and III.It has been suggested that factor I might be equivalent to copolymerase III. DNA polymerase III* is a multimeric form of DNA polymerase III protein. Chain elongation takes place in short Okazaki fragments which are then covalently linked to form a continuous strand.
It results in the formation of the RFII structure, which consists of a circular viral (+) strand and a complementary (-) strand which is not covalently continuous. Unwinding protein is also required during chain elongation.