dsRNA Phages - øX174 ICOSAHEDRAL - ssDNA Phages

Bacteriophages - Part One
Bacteriophages - Part Two
Types of Phages
ssDNA Phages
dsDNA Phages
ssRNA Phages
dsRNA Phages - øX174 ICOSAHEDRAL ssDNA Phage
Capsid of øX174
DNA of øX174
Proteins of øX174
Infective Cycle - Adsorption
Infective Cycle - Eclipse
Genes and Proteins oføX174
Parental RF Formation (ssdNA --> RF)
Presynthesis Stage
RNA Primer Synthesis
Chain Elongation
Gap Closure
RF Replication
Reciprocating Strand Model
Rolling Circle Model
Single Stranded DNA Synthesis (RF --> SS)
Ff Phages - HELICAL ssDNA Phages
Capsid Proteins of Ff Phages
DNA of Ff Phages
Life Cycle of Ff Phages
Attatchment of the Phage to the Bacterial Cell
Entry of the Phage into the Host Cell
Formation of Replicative Form(SS --> RF)
RF Replication (RF --> RF)
Single Strand Synthesis(RF -->SS)
Maturation of the Virus Particle
Phage T4 - Virulent dsDNA Phage
Parts of Phage T4 - Virulent dsDNA Phage
DNA of Phage T4
Phage T4 Multiplication Cycle
Adsorption of Virion to Host Cell
Penetration of Nucleic Acids
Protein Synthesis of Phage T4
Breakdown of the Bacterial Chromosome
Arrest of Host Cell Development
Classes of T4 Mutation / Replication of DNA
Enzymes of DNA Precursor Synthesis
Nucleases in Phage T4

dsRNA Phages - øX174 ICOSAHEDRAL - ssDNA Phages

dsRNA Phages - øX174 ICOSAHEDRAL - ssDNA Phages - The E. coli phage φX174 is the best known of the ssDNA phages. It has a particle weight of 6.2 x 106 daltons and sediments at 114S.

The diameter of the particle without its spikes has been variously reported as 24-25 nm (negatively stained particles), 29 nm (light scattering data) and 31.4+-0.6 nm (calculated from the translational diffusion coefficient determined by laser self beat spectroscopy.

Endonucleases
Exonuclease
Deoxycytidine and Deoxyuridine tri-and diphosphatase (56)
Thymidylate Synthetae(td)
Dihydrofolate Reductase (frd)
Ribonucleoside diphoshate Reductase(nrd)
Deoxycytidylate Hydroxymethylase (42)
Deoxynucleotide kinase(I)
Deoxycytidylate Deaminase (cd)
Endonuclease II
Endonuclease III
Endonuclease IV
Endonuclease V
Endonuclease VI
Endonuclease A
Endonuclease B
DNA Polymerase
DNA Ligase
Polynucleotide Kinase
DNA Phosphates
DNA Dependent ATPases
RNA Ligase
DNA Glucosyltransferases
DNA Methylase
Enzymes of Nucleic Acid Synthesis
Priming of Synthesis
Elongation
DNA Binding Protein
Okazaki Fragments
Rolling Circle Mechanism
Recombination
Maturation of Infective Progeny
Head Assembly of Phage T4
Prohead I(400S) of Phage T4
Prohead II (350S) of Phage T4
Prohead III (550S) of Phage T4
Mature Head (1100S) of Phage T4
Tail Assembly - Base Plate of Phage T4
Core Tube and Sheath of Phage T4
Tail Fibres of Phage T4
Assembly of Tail Fibres

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