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Index >> Bacteriophages - Part One >> Parental RF Formation (ssDNA to RF )

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Parental RF Formation (ssDNA to RF )


Parental RF Formation (ssDNA to RF ) - Viral ssDNA (24S) on entering the host cell synthesizes a complementary strand, and is converted into the duplex state replicative form (RF). RF DNA exists in two forms, RFH and RFI. RFII(16S) is the earlier form in which at least one of the strands is not covalently continuous, usually because of the presence of a nick or gap.


Closure of the gap results in RFI (21 S), which is in the form of a supercoiled, covalently dosed duplex circle. It consists of one viral or possible strand (v-strand) and one complementary or negative strand (c-strand). Synthesis of the RF form does not require any phage specified protein, and depends entirely on host function.


Synthesis of RF DNA from viral ssDNA may be divided into the following stages:

(i) presynthesis stage,

(ii) RNA primer formation,

(iii) elongation of a DNA chain from the primer and

(iv) gap closure to form a covalently closed circular strand.
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