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Gene Exchange in Bacteria

Gene Exchange in Bacteria
Gene e change in bacteria takes place by processes known as transforma­tion, transduction and conjugation. The addition of foreign DNA to ac­tively growing bacterial culture results in chance entry of foreign DNA into cells by modification of bacterial cell envelope followed by the intake of DNA into the bacterial genome.

Transduction involves bacteriophages or bacterial viruses whose DNA enters the bacterial cell followed by the disintegration of the bac­terial chromosome. The phage DNA multiplies in the cells, the cell walls undergo lysis releasing the phages which have multiplied in the mean time.

The phages which mediate this type of transduction are known as lytic or virulent phages. On the contrary when a temperate phage (non­virulent type) enters the bacterium, the phage DNA becomes attached to the bacterial chromosome and remains integrated with the bacterial genome for many generations. This process is also known as lysogeny. At times, temperate phages may turn virulent leading to lysis and produc­tion of more bacteriophages.

The temperate phage, when freed from the cell may carry with it small pieces of DNA which upon delivery to a next host cell can add an additional character to the new cell's capabilities.

Contact between two cells is required for conjugation, one acting as a donor and the other a recipient. The donor possesses a fertility factor (F+) and the recipient has no such factor (F- but must be viable for successful conjugation.

 

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