Index >> Biotechnology in Agriculture >> Procedures for Agrobacterium Mediated Transformations

Procedures for AgrobacteriumMediated Transformations
Several procedures have been followed till to date: (a) through conven­tional gall formation on wounded plant stems or .leaves that are not amenable to monitoring with disarmed vectors, (b) Co-cultivation of Agrobacterium with plant protoplasts, a method that has been successful in plants where regeneration of plants from protoplasts has proved to be successful and (c) tissue transformation with ex-plants (leaf, cotyledon sec­tion or somatic embryos) inoculated with Agrobacterium, a procedure that has proved faster than protoplast regeneration.