Virus Resistance in Transgenic Plants

Biotechnology in Agriculture
The New Green Revolution
Nucleic Acids
Protein Synthesis
Southern and Northern Blot Techniques
Nucleic Acid Hybridization
Recombinant DNA Technology and Gene Cloning
Some Restriction Enzyme Sequences the Arrow Indicates Actual Clevage Site
Gene Exchange in Bacteria
The Agrobacterium Mediated Transfer of Genes
Procedures for Agrobacterium Mediated Transformations
Direct DNA Transfer Technologies
Antisense RNA Strategy
Frost Control Biotechnology
Virus Resistance in Transgenic Plants
Transformation of Sequences Related to Viral Capsid Protein
Resistance Conferred by Antisense RNA
Antibody Mediated Resistance

Virus Resistance in Transgenic Plants

Virus Resistance in Transgenic Plants
There have been many reports about the development of transgenic plants which have become resistant to virus infections. These plants showed resistance to virus infections when they were transformed with sequences related to several gene functions.

Some of the areas where successes have been seen related to sequences concerning viral capsid protein, viral movement protein, antisense RNA, antibody-mediated resistance, interferon-related genes and host genes involved in plant protection.

Viruses are biochemical complexes consisting of a RNA or a DNA genome packaged into a protein capsid which mayor may not be surrounded by a membrane envelope. The protein coat 'covered genome is referred to as the nucleocapsid. The proteins on the surface of the capsid and envelope determine the interaction of the virus with the host and elicit the protective immune response against the virus. Some virus particles also contain enzymes required to facilitate the replication of the virus.

The tobacco mosaic virus (TMV) is an example of a virus with helical symmetry whose capsomeres (many protein subunits of the capsid) appear as projections that are assembled on the RNA genome into rods extending to the length of the genome.

In other viruses the capsomere arrangement is cubical or icosahedral enclosing its nucleic acid component. Engineering resistance in plants involves either countering the capsid properties or disrupting the virus replicating mechanisms in the hot.

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