Culture Techniques
The
recognition
that
microorganisms
in
nature
always
exist
in
a
mixture
demanded
that
for
the
study
of
various
physiological
types,
pure
cultures
were
essential.
Realising
this,
S.Winogradsky
(1856.
1953)
and
M.W.
Beijerinck
(1851-1931)
developed
the
technique
of "enrichment
culture".
Basically
it
uses
the
principle
of
natural
selection.
When
a
culture
medium
of
a
defined
chemical
composition
is
inoculated
with
a
mixed
microbial
population,
only
the
organism
to
which
the
environment
is
ideal
will
rapidly
outgrow
the
others
by
repeated
subculturing
into
new
medium
of
the
same
composition
it
is
possible
to
isolate
a
pure
culture.
