Effect of Temperature on Enzyme Activity - Enzyme action is greatly affected by temperature. If the temperature is increased by 10°C the rate of most chemical reactions is doubled. However, at 40°C 60°C there is loss of enzyme activity because denaturation of proteins occurs at this temperature.
pH: At optimum pH the activity of enzymes is maximum. For most enzymes the effective pH range is 4-9. Beyond these limits denaturation of enzymes takes place. The optimum pH for pepsin is 2.0 and for trypsin 8.
Ions: Enzyme activity is affected by H ion concentration and their ionic concentrations. Some enzymes require a loosely bound cation such as Mg++