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Index >> Enzymes,Isozymes and Coenzymes >> Effect of Temperature on Enzyme Activity

Effect of Temperature on Enzyme Activity

Effect of Temperature on Enzyme Activity -

Enzyme action is greatly affected by temperature.

If the temperature is increased by 10°C the rate of most chemical reactions is doubled.

However, at 40°C­ 60°C there is loss of enzyme activity because denaturation of proteins occurs at this temperature.

pH: At optimum pH the activity of enzymes is maximum.

For most enzymes the effective pH range is 4-9. Beyond these limits denaturation of enzymes takes place.

The optimum pH for pepsin is 2.0 and for trypsin 8.

Ions:

Enzyme activity is affected by H ion concentration and their ionic concentrations.

Some enzymes require a loosely bound cation such as Mg++

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