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Bacterial Food Infections

Bacterial Food Infections - Bacterial food infections include salmonellosis, Clostridium perfringens gastroenteritis, Vibrio parahaemolyticus infection etc.

Salmonellosis is an infection resulting from ingestion of the vegetative cells of Salmonella and is the most frequently occurring bacterial food infection. Salmonella infection can be caused by a large number of species and serotypes of Salmonella. These bacteria grow well in the food at room temperatures and have a pH range optimum for growth between 4.1 to 9.0. Thus, they can also grow in low acid foods.

The lowest aw for growth varies with the food but varies from 0.93 to 0.95. These organisms can attain considerable numbers causing detectable changes in appearance, odour or taste of foods in which they grow. Infection follows consumption of the food.
Both animals and human beings are directly or indirectly the sources of contamination of food with Salmonella. The organisms may come from either actual cases of disease or from the carriers. The organisms may also come from cats, dogs, chickens, cattle and other animals.

Individuals differ in their sensitivity to Salmonella infection but in general, morbidity is high in any outbreak. Susceptibility varies with the species, strains and the total number of bacteria ingested. The incubation period usually is between 4-36 hours compared to 2-6 hours in the case of staphylococcal poisoning. Typical symptoms of Salmonella gastrointestinal infections are nausea, vomitting, abdominal pain and diarrhoea that usually appear suddenly and which may be preceded by headache and chills. Mortality is low and the severity and the duration varies with the number of bacteria ingested. Usually symptoms persist for 2-3 days. Salmonella out breaks can be prevented by avoiding consumption of contaminated food, destruction of Salmonella by heat or by the prevention of Salmonella growth by refrigeration or by other means.

E. coli is generally regarded as a part of the natural flora of the human and animal intestinal tract. However, in recent years, several serotypes of E. coli have been implicated in human and animal diar­rhoeal diseases. The organisms responsible for such food poisoning outbreaks have been designated as enteropathogenic E. coli (EEC) . Some of these EEC produce an enterotoxin which causes cholera like or enterotoxigenic illness in humans. This can be serious specially in children. Following ingestin of these organisms, the bacteria colonize the upper intestine and produce an enterotoxin which apparently mediates the movement of water in the intestinal lumen. Such fluid accumulation occurs in the absence of invasive bacteria.

Other groups of E. coli that cause colitis (dysentry like syndrome) are nonenterotoxigenio but penetrate the epithelial cells of the colonic mucosa. For either the enterotoxigenic or the invasive illness to occur a large dose of the EEC is required. Therefore, foods which are highly contaminated or inadequately preserved allow the growth of these organisms. These organisms al e relatively heat sensitive and can be readily destroyed by pasteurization or by proper cooking methods.

Vibrio parahaemolyticus gastroenteritis is also a commonly occurring food intoxication. This organism which is a Gram-negative, curved rod shaped and motile will grow at seven per cent salt concentration with an optimum temperature of 35-37°C. This has been isolated from many sea foods but can be killed by proper cooking.

Other organisms implicated in food borne disease outbreaks are species of Yersinia, Arizona, Streptococcus pyogenes and S. faecalis. The food infections caused by these result from the growth and increase in the dosage of the pathogen. Some of these organisms can tolerate a salt concentration of 5-6 per cent. However, no enterotoxin production by these organisms has been so far demonstrated.

Among the spore forming bacteria that cause food infections are the Clostridium perfringens and Bacillus cereus. Clostridium perfringens gastroenteritis is caused by C. perfringens (welchii) which is a Gram positive, anaerobic, nonmotile spore former with an are optimal growth temperature of 37-43°C. Spores of this organism are heat resistant and are' found in raw food, soil, sewage and animal faeces. Most commonly, meat that has been cooked and allowed to cool slowly before consumption permits the growth of these organisms. Cooking destroys only the vegetative cells and during cooling, germination and growth of spores occurs, if the food is not adequately refrigerated.

Symptoms of the disease which appear following ingestion of the food in 8-24 hours are acute abdominal pain and diarrhoea. Fever and nausea are rare. Ingestion of millions of viable cells ore required for symptoms to appear. The enterotoxin is released in the gut during sporulation and results in excessive fluid accumulation in the intestinal lumen. The enterotoxin is relatively heat sensitive and is inactivated at 60°C for 10 minutes. Prevention of outbreaks includes rapid cooling of cooked meats and other foods and reheating of left overs before consumption.

Bacillus cereus which is a Gram positive, aerobic spore forming rod-shaped organism has been reported as an etiologic agent in numerous food poisoning outbreaks. The spores are heat resistant and survive considerable degree of cooking. For symptoms to develop, large number of cells have to be ingested. The mechanism of pathogenicity is believed to involve lysis of the bacterial cells in the intestinal tract and the release of enterotoxin which appears to be a protein.


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