Microbiology Procedure
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Number of Nucleotides in Average Gene

Number of Nucleotides in Average Gene

It is possible to find out the number of nucleotides in a gene if the molecular weight of the gene is known, since each base pair has a molecular weight of about 600.

Thus in the bacteriophage T4, which has over a 100 genes and a chromosomal molecular weight of 120 million, each gene has an average MW of 1.2 million.

Now, since the molecular weight of a pair of bases is about 600 the average gene should contains about 2,000 base pairs. However, all the genes of T4 are not known.

Assuming that the total number of genes will at maximum be doubled, the average gene would have 900-­1500 base pairs

The F2 RNA virus contains only 3,000 nucleotides which can code for about 1,000 amino acids. The tobacco necrosis virus contains still fewer nucleotides, about 1000, which are just sufficient to code for the average sized protein molecule.

The necrosis virus invades a host cell along with another RNA virus (with about 6,000 nucleotide), and uses the enzymes of the larger virus for its replication.

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