Microbiology Procedure
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Index >> Growth of Microorganism >> Synchronous Growth

Synchronous Growth


Synchronous Growth
- A growing microbial culture contains cells dividing asynchronously and the properties of the population are the average properties of the individual cells.

While studying cell cycle events we want to measure changes in the biochemical features of individual cells and we therefore need to amplify the physiological events by producing a synchronously dividing culture in which all the cells divide at roughly the same time.
There are two methods available for producing the synchronous cultures.

1. Induction methods - They rely on synchronising an exponential phase culture by appropriate and usually sudden changes in the environment, such as alteration in temperature, concentration of nutrients or illumination for photoautotrophs.

2. Selection methods - The cells are physically separated from an exponential-phase culture at a particular point in the growth cycle.
The methods include
(i) centrifugation on a density gradient
(ii) filtration of cells through a cellulose nitrate filter and inverting it and passing medium through the filter from above. When cells divide they fall off the filter giving a continuous supply of newly born cells

(iii) the filtration of cells of certain sizes or the selective adsorption of cells on surfaces.

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