Microbiology Procedure
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Rotation of Carrier

Rotation of Carrier
In this scheme the carrier molecule is believed to have a diameter nearly equal to the thickness of the membrane. The substance to be transported binds to the carrier molecule, forming a carrier substrate complex

This rotates through 180° and releases the substance on the other side of the membrane

Jacques Monod and his associates studied the transport of lactose (milk sugar) across the cell membrane of the bacterium E. coli. This bacterium accumulates lactose 500 - 1000 time against the Concentrations gradient

A 'revolving door' model of active transport has be proposed to explain the passage of lactose through the cell membrane. The carrier, a protein, is visualized as a revolving (rotating) door in the cell membrane.  

The carrier protein has a slot which normally faces outside, into which fits the substance to be transported. The carrier protein is believed to undergo a change in shape (conformational).

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