Microbiology Procedure
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Viruses

Viruses - They are a cellular, infectious agents. Since they have no associated metabolism one can argue whether they are organisms at all. They have no such activity except replication, that too only in cytoplasm of live cells. They consist of a fragment of nucleic acid (DNA or RNA, never both) surrounded by a layer of protein. They cause important diseases.

Viroids
are minute infectious agents of plants which are composed of single naked RNA molecules.
The first report of these pathogenic RNA molecules in the early 1970s aroused much interest, since it was previously thought that infectious nucleic acids could survive outside a cell only if they are encapsulated in a protein capsid.

Viroid RNAs are very unusual: they are circular, uniquely folded and so small that the largest one so far described (CEV: citrus exocortis viroid) is only 371 nucleotides long about 1/10h of the size of the smallest RN A virus. Viroids so far are known to cause diseases in plants only.

Prions (proteinaceous infective particles) or slow viruses are a group of recently discovered infectious particles. They are associated with certain degenerative diseases of the central nevous system such as kuru, a disease restricted to a few cannibalistic tribes of New Guinea and transmitted by eating uncooked brain, and other diseases such as scrapie of sheep and goats and Crutzfeldt Jacob disease of humans and animals. Prions appear to be proteins or glycoproteins with no associated nucleic acid of any kind.

Since replication of the prion protein presumably occurs by normal process, this implies that these proteins may be able to bring about the production of their own requisite mRNA

If this were, so, prions would have revolutionary impact on molecular biology since they would constitute the only example

where genelic information passes from protein to nucleic acid and not the other way around, However, recent evidence shows that scrapie glycoprotein may be a product of a normal host gene, indicating that these infectious agents may be more conventional than thought previously.

 

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