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Index >> Microbes and Atmosphere >> Centrifugation

Centrifugation

Centrifugation
Centrifugal samplers use circular flow patterns to increase the gravitational pull within the sampling device in order to deposit particles. The most common is the Reuter's air sampler which is based on the centrifugal force. Air is sucked in through the propeller blades and as it traverses through the body of the sampler, it gets deposited onto the thin agar media which lines the inner wall of the sampler. After a known period of time the media is taken out and incubated for further studies.

SKC aluminium cyclone
A - Air is Drawn into the Sampler
B - Return air Flow

There is another device based on this principle, the cyclone which is a tangential inlet and return flow sampling device. These samplers are able to sample a wide range of air volumes (1-400 L/ min), depending on the size of the unit. The unit operates by applying suction to the outlet tube, which causes air to enter the upper chamber of the unit at an angle. The flow of air falls into a characteristic tangential flow pattern which effectively circulates air around and down along the inner surface of the conical glass housing. As a result of the increased centrifugal forces imposed on particles in the air stream, the particles are sedimented out. Analysis is performed by rinsing the sample with an appropriate liquid medium, collection of the medium and subsequent assay by standard methodologies.

 

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