Microbiology Procedure
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Microbes and Chemical Changes

At about this time Liebig the German chemist, had claimed that fermentation and putrefaction were processes caused by the chemical instability of organic molecules. On the other hand, Pasteur had shown that living yeast cells were required for the conversion of sugar into alcohol and had suggested that these fermentations, and transformations are brought about by the agency of microorganisms. At this time. the distilleries in France, where alcohol was produced from sugar beet had difficulties in their fermentations and requested Pasteur for help. Pasteur found that the trouble was due to the replacement of the alcoholic fermentation by a lactic fermentation. Instead of finding yeast in the fermenting brew, he found rod-shaped structures.

He concluded that these rod shaped organisms were responsible for the conversion of sugar into lactic acid. Subsequently, he also showed that different fermentations are brought about by specific microorganisms.

For example, the alcoholic fermentation was the result of living yeast cells while the lactic acid fermentation was the result of the activity of lactic bacteria.

Interestingly in 1897, after Pasteur, Buchner showed that intact yeast cells were not. required for the conversion of sugar into alcohol and carbon dioxide He prepared a cell free yeast juice by grinding yeast cells with sand and showed that this cell free juice had the ability to carry out the alcoholic fermentation. He concluded that the active agent in the yeast cells responsible for bringing about the fermentation was a protein and he named it as zymase. This finding gave support to Liebig's thesis that living cells were not required to bring about fermentations and marks the beginning of the study of enzyme chemistry.

 

 

 

 

 

 

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