Microbiology Procedure
  Home  Link to us  Resources  Site map  Search  Language

Index >> Microbial Control >> Antibiotic Sensitivity Assay Agar Diffusion Method

Antibiotic Sensitivity Assay Agar Diffusion Method

Antibiotic Sensitivity Assay Agar Diffusion Method -

The principle used here is that antibiotic will diffuse from a paper disc or small cylinder into an agar medium that contains test organisms.

Inhibition is observed as a failure of the organism to grow in the region of the antibiotic. A common application of this method is the Kirby Bauer test, developed in the 1960s.

The procedure is used to determine the sensitivity of an organism isolated from a patient to a series of antibiotics.

The results serve a guide to physician to prescribe a drug.

The results serve a guide to physician to prescribe a drug. An agar medium such as Mueller Hinton medium is inoculated with the organism and poured to the plate.

Paper discs containing known concentrations of antibiotics are applied to the surface, and the plate is incubated. The appearance of a zone of inhibition surrounding the disc is indicative of sensitivity.

By comparing the diameter of the zones to a standard table, one may determine if the test organism is susceptible, or resistant to the antibiotic.

If the organism is susceptible, it is likely to be killed in the blood stream of the patient if that concentration of the drug is reached. Resistance indicates that the antibiotic will not be effective at that concentration in the blood stream.

Home | Site map | Submit Article | Resources | Search