Microorganism Controlling by Auto Claves

Microorganism Controlling by Auto Claves

Microorganism Controlling by Auto Claves -

The principle used here is to increase the pressure of steam (gas) in a closed system that increases its temperature.

The water molecules become more aggregated that increases their penetration considerably.

This principle is used to reduce sterilising time in the laboratory instrument called the autoclave.

It contains a sterilising chamber into which articles are placed and a steam jacket where steam is maintained.

A special valve increases the pressure to 15 lb/sq. inch above normal atmosphere pressure.

The temperature rises to 121.5°C and super heated water molecules rapidly conduct heat into the microorganisms within about 15 mts.

Culture media, glassware, metalware etc. are sterilised by this method.

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