Classification
of
Restrition
Enzymes
Restriction enzymes (endonuclease) recognize specific nucleotide sequences in double stranded DNA (dsDNA) and cleave both strands. Over 150 specific endonucleases have been discovered which recognize more than 40 different specific sequences.
Although all restriction enzymes recognize specific nucleotide sequences, not all cleave DNA at specific sites. Two classes of restriction enzymes are recognized, Class I Enzymes and Class II Enzymes
