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Induction of Selcetive Mutations

Induction of Selcetive Mutations
Physical and chemical mutagens cause random mutations and as a consequence obtaining a desired mutation is difficult and time consuming. The discovery of transposable genetic elements (see later) has enabled the isolation of selective mutations.

This is possible because some of the transposable elements carry along with them markers which allow selection such as resistance to antibiotics which allows easy detection. Thus, by use of transposable elements, not only mutations can be induced but also selected in any microbial function.

The Mu bacteriophage has also been used as a mutagenic agent because of its ability to integrate itself with any part of the bacterial genome and thereby cause mutations. Recently, a technique by which synthetic nucleotide blocks can be inserted into different regions of gene sequences (block mutations) such as initiators, initiators etc. has been developed. This technique is useful in understanding the functions of DNA sequences in genes which cannot be understood otherwise.

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