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Recombination in Eukaryotes

Recombination in Eukaryotes
Unlike prokaryotes, in eukaryotes the genes are organised onto several chromosomes which are present in the nucleus. The number of chromosomes is species specific and is a stable character of that species. Genetic recombination here is essentially a sexual process involving recombination during meiosis leading to the formation of male and female haploid gametes. Fusion of gametes results in the new types of diploids. However in the fungi there is some complexity. Unlike most eukaryotes, some only exist in the haploid state.

Consider the life cycle of two fungi. Aspergillus nidulans and Saccharomyces cerevisiae. In A. nidulans, haploid mycelia of different strains fuse and form fruit body. In this fruit body there is karyogamy and meiosis, During meiosis the chromosomes from the two nuclei pair up and recombine with each other to give hybrid chromosomes which carry genes from both parent chromosomes.

Meiosis is followed by the formation of spores which germinate to produce haploid mycelium.

However, S. cerevisiae is non mycelial. The diploid cells formed after fusion can undergo normal growth and division. There are no fruit bodies, and each diploid cell can undergo meiosis to produce haploid cells.

In eukaryotic microbes, there is alternation of a haploid and a diploid generation. In different forms, there is a wide variation in the relative importance of the haploid and diploid phases for vegetative reproduction and in the degree of sexual differentiation they exhibit

In fungi, the predominant phase may be haploid or diploid or there may be roughly equal role of both; there may be morphologically distinct male and female forms or there may be sexual conjugation between two similar cells derived from the same clone.

An important characteristic of eukaryotes is the presence of extra-nuclear DNA in organelles such as chloroplasts and mitochondria. Eukaryotic cells may also possess plasmids. S. cerevisiae has a 2µm plasmid that has formed the basis of gene cloning in yeast both in its own right and as a hybrid molecule with bacterial plasmids allowing the analysis of yeast genes both in bacteria and in the yeast it self.

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