Direct
Counting
of
Cells,
Spores,Viable
Plate
Count
There are several methods that can be used for counting
the
cells
and
spores
of
bacteria
and
other
microbes. These include direct count, plate count, and most probable number (MPN) determinations.
(i) Direct
count
The
density
of
cells,
spores
etc.
of
microorganisms
can
be
found
out
by
counting
their
number
in
a
unit
volume.
The
simplest
technique
is
to
use
a
special
counting
chamber,
of
a
type
that
used
in
a
haemocytometer
for
counting
the
blood
cells.
The
counting
chamber
is
simply
a
ruled
slide
with
a
supported
glass
cover
that
holds
a
definite
volume
of
fluid.





