Enrichment
cultures
This
method
is
used
for
those
microbes
which
are
in
small
numbers
in
the
sample
and
whose
growth
is
slow
than
other
species
present.
Some
bacteria
have
very
specific
requirements
for
their
growth.
The
principle
of
enrichment
culture
is
to
control
the
nutrients
and
culture
conditions
(temperature,
air
supply,
light,
pH
etc.)
in
such
a
way
that
it
suits
only
to
the
given
species.
If
a
medium
containing
salt
solution
with
NaNO2
at
pH
8.5
is
inoculated
with
a
sample
of
garden
soil
and
incubated
in
air
in
dark
at
25-30°C,
pure
colonies
of
Nitrobacter
can
be
obtained.
Cultures
of
other
soil
bacteria
may
be
selectively
enriched
by
varying
the
composition
of
the
medium
and
the
conditions
of
incubation
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