Microbiology
/Microbiological
Methods
It
must
be
evident
from
the
history
of
microbiology
how
important
is
the
suitable
methodology/methods
in
development
of
a
discipline.
Three
techniques
in
particular
are
to
be
mastered
before
the
science
of
microbiology
could
evolve
beyond
a
primitive
visionary
state.
1.
Microscopy.
Since
microbiology
is
mainly
concerned
with
the
study
of
living
organisms
of
microscopic
dimensions,
its
development
depended
entirely
upon
the
refinement
of
the
microscope.
2.
Sterilisation
Methods -
In
order
to
grow
a
particular
microorganism,
the
medium
used
had
to
be
freed
from
all
other
living
organisms
i.e.
sterilisation
methods
had
to
be
developed.
3. Pure
Culture
Methods
- Once
it
was
possible
to
obtain
suitable
sterile
growth
media,
it
became
practicable
to
introduce
methods
to
separate
different
microbes
from
each
other
and
to
maintain
them
in
pure
culture.
Then
could
be
studied
their
individual
characteristics.
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