Pour
Plate
Method
The
sample
should
be
diluted
successively
with
sterile
water.
The
agar
medium
is
maintained
in
molten
state
at
45° C.
One
ml
of
each
dilution
is
added
to
each
sterile
Petri
dish
to
which
is
then
poured
9ml
of
sterile,
cool
agar
medium.
The
contents
are
thoroughly
mixed,
and
allowed
to
solidify.
The
dishes
are
incubated
at
suitable
temperature.
After
few
days,
different
kinds
of
microbe:
grow
as
separate
colonies.
Cells
from
individual
colony
may
be
picked
up
for
a
subculture.
