Simple
Staining
Techniques/Methods/Procedure
A
small
amount
of
bacteria
is
placed
in
a
droplet
of
water
on
a
glass
slide
then
it
is
air
dried.
The
slide
is
passed
through
a
flame
in
a
process
called
heat
fixing,
which
fixes
the
cells
to
the
slides,
kills
most
organisms
and
prepares
them
for
staining.
Now
the
slide
is
flooded
with
a
basic
dye
such
as
crystal
violet
or
methylene
blue
for
a
minute
or
so.
The
positive
charged
dye
is
attracted
to
the
bacterial
cytoplasm
which
has
a
negative
charge,
and
staining
takes
place.
This
is
effective
for
vegetative
cells,
the
stains
do
not
easily
penetrate
spores.
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