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Synthetic / Defined Culture Media

Synthetic or Defined Culture Media

Synthetic or Defined Culture Media consist wholly of dilute, reproducible solution of chemically pure, known inorganic and/or organic compounds. Artificial media of exactly known, reproducible composition are called synthetic or chemically defined media. The formulation and use of these media requires an exact knowledge of the nutritional requirements of the microorganism to be cultivated.

A simplest inorganic synthetic culture medium for common, sulphur oxidising, chemolithotroph, soil bacterium, Thiohacillus thiooxidans is as follows: In modem laboratories, dehydrated media are used.

Commercially avail able portable, dehydrated, powdered, bottled mixtures of a great variety of dehydrated media or their ingredients are used. These are prepared simply by adding weighed portions of the ingredients to the required amount of water.

The synthetic media may be a general purpose medium used for majority of microbes or selective media used for a selected microbe or differential media used for differential isolation of microbes in same sample. We describe these media later under pure culture methods.

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